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BEtarget: a quick guide-RNA designer for base editing by CRISPR CBE and ABE
PAM type:
SpCas9 from Streptococcus pyogenes: 5'-(target)NGG-3'
SpCas9-NG from Streptococcus pyogenes: 5'-(target)NGN-3'
FnCpf1 from Francisella: 5'-TTN(target)-3'
AsCpf1 from Acidaminococcus or LbCpf1 from Lachnospiraceae: 5'-TTTN(target)-3'
User defined
*
Or define your own PAM:
PAM sequence:
PAM position:
3'
5'
Target site length (nt):
Base editing type:
C to T
A to G
Base editing window:
to
Target (reference) genome:
Oryza sativa japonica (Nipponbare, IRGSP1.0)
Oryza sativa japonica (Nipponbare, MSU7.0)
Oryza sativa Indica (93-11, ASM465v1)
Oryza sativa indica (MH63RS3)
Oryza sativa indica (ZS97RS3)
Oryza sativa indica (MH63)
Oryza sativa indica (ZS97)
Oryza sativa indica (Shuhui498, R498)
Arabidopsis thaliana (TAIR10)
Arabidopsis lyrata (v.1.0)
Ananas comosus v3 (Pineapple)
Apple (GDDH13 Version 1.1)
Brachypodium distachyon (v1.0)
Brassica napus (Genoscope, v5)
Brassica rapa (IVFCAASv1)
Brassica oleracea var. oleracea (NCBI)
Camelina sativa (false flax, NCBI)
Cyanidioschyzon merolae (ASM9120v1)
Cucumber (ChineseLong v2, cucurbitgenomics)
Cucurbita moschata (Rifu), CuGenDB v1
Glycine max (v1.0)
Gossypium hirsutum (v1.1, Upland cotton, NBI-AD1)
Hordeum vulgare (ASM32608v1)
Kiwifruit (KIR)
Dimocarpus longan
Medicago truncatula (MedtrA17_4.0)
Musa acuminata (MA1)
Mguttatus_256_v2.0, Mimubase
Morus notabilis (NCBI)
N. tabacum K326 Sierro (Tobacco, Sol Genomics)
Solanum tuberosum (SolTub_3.0)
Solanum tuberosum (Potato, A6-26)
Physcomitrella patens (PhytozomeV11)
Populus trichocarpa (JGI2.0)
Prunus persica (Prupe1_0)
Prunus persica V2 (Prupe V2, GDR)
Setaria italica (JGIv2.0)
Solanum lycopersicum (SL2.50)
Sorghum bicolor (Sorbi1)
Theobroma cacao
Vitis vinifera (IGGP_12x)
Zea mays (AGPv4)
Zea mays (B73_v5)
Zoysia japonica (Zoysia Genome Database, r1.1)
Zoysia matrella (Zoysia Genome Database, r1.1)
Zoysia pacifica (Zoysia Genome Database, r1.1)
Human (GRCh38.p10)
Mouse (GRCm38.p5)
Zebrafish (GRCz10)
Drosophila melanogaster (FlyBase; Ensemble BDGP6)
Caenorhabditis elegans (WormBase; Ensembl WBcel235)
None
Arabidopsis thaliana mitochondrion (TAIR10)
Oryza sativa mitochondrion (Nipponbare)
Zea mays mitochondrion (B73v2)
Human mitochondrion (GRCh38)
Mouse mitochondrion (GRCm38)
Zebrafish mitochondrion (GRCz10)
Arabidopsis thaliana chloroplast (TAIR10)
Oryza sativa chloroplast (Nipponbare)
Zea mays chloroplast (B73v2)
* If the reference genome sequence is not available, you can select a close relative species or "None".
Sequence submission
You can use the supporting tool (
GeneCat
,
http://skl.scau.edu.cn/genecat/
) to obtain the fully genomic sequence and CDS of your target gene.
If the genomic sequence and coding sequence (CDS) of your target gene is submited, the target gene is based on this sequence, and the gene ID is not needed.
Please enter the
genomic sequence
of your target gene (<10kb).
See example
.
Please enter the
CDS sequence
of your target gene (<10kb).
See example
.
Or
Input your target gene ID of the selected reference genome
(If a gene ID is submited, candidate targets are based on the reference genome, and the above sequence submission is not needed.)
Gene locus:
eg:
Os01g0104000
or LOC_Os01g01410
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