To prepare CRISPR/Cas9 binary constructs, the first step is to generate target-sgRNA expression cassette(s). The primerDesign-V (Vector) tool automatically outputs the primers for PCR-based generation of the sgRNA expression cassettes in the vector system of our lab and other vectors using the adapter-ligation method.

The primerDesign-A (Amplification) is used to design specific PCR primer sets for amplification of a genomic sequence containing the target editing site(s) and for sequencing (A), or for amplification of any genomic sequence (B) .