primerDesign-V: automatically generates primers for vectors constructing of CRISPR/Cas9 genome editing in plants

Currently, the primerDesign-V tool can design primers for preparation of the sgRNA expression cassettes in the vector system of our lab and other vectors using the adapter-ligation method.


Please select the vector system for construction of sgRNA expression cassette:


Reference:
Ma X, Zhang Q, Zhu Q, et al., A robust CRISPR/Cas9 system for convenient high-efficiency multiplex genome editing in monocot and dicot plants.
2015, Mol. Plant, 8 (8), 1274-1284.
[Link]
Ma X, Liu Y-G*. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol., 2016, 115, 31.6.1-31.6.21. [Link]


 Please enter your target sequence (20 nt) without PAM. [..demo]

ID Target sequence of CRISPR/Cas9
(5'-3')
Promoter Method Primers(5'-3') Tm
1
2




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