MMEJ-KO

MMEJ-KO: a tool for automatically designing paired guide-RNAs for MMEJ-mediated fragment deletion using CRISPR/Cas genome editing.

In addition, we provide a tool, repFinder, for finding identical direct and inverted repeats of DNA sequence.

PAM type:

* Or define your own PAM (please select "User defined"):
	PAM sequence:
	PAM position:
	Target site length (nt):

Target (reference) genome:
* If the reference genome sequence is not available, you can select a close relative species or "None".

Sequence submission

Please enter the sequence of your target gene or fragment (genomic sequence). See example.

The length of sequence should not be more than 10 kb.

Or input your gene locus ID

Gene locus	Upstream flanking (0-5000 bp)	Downstream flanking (0-1000 bp)

eg: Os01g0131300 or LOC_Os01g04010

Microhomology repeat setting (option)

Minimum repeat size (3 bp):
Desirable deletion including the region (bp):	From		to
	eg.	500		700

If you use MMEJ-KO, please cite:

Xie X, Liu W, Dong G, Zhu Q, Liu Y-G. 2021. MMEJ-KO: a web tool for designing paired CRISPR guide RNAs for microhomology-mediated end joining fragment deletion. Sci. China Life Sci.. (DOI: 10.1007/s11427-020-1797-3)