targetDesign: a quick guide-RNA designer for CRISPR/Cas9/Cpf1 genome editing

  PAM type:
   * Or define your own PAM:
PAM sequence:
PAM position:
Target site length (nt):

  Target (reference) genome:
    * If the reference genome sequence is not available, you can select a close relative species or "None".

Sequence submission
Please enter the sequence of your target gene or fragment (genomic sequence). See example.
The length of sequence should not be more than 10 kb.
Or input your gene locus ID

Gene locus
Upstream flanking
(0-5000 bp)
Downstream flanking
(0-1000 bp)

eg: Os01g0131300 or LOC_Os01g04010

Copyright © The Genetic Engineering Laboratory of South China Agricultural University