primerDesign-V: automatically generates primers for vectors constructing

primerDesign-V: automatically generates primers for vectors constructing of CRISPR/Cas9 genome editing in plants

Currently, the primerDesign-V tool can design primers for preparation of the sgRNA expression cassettes in the vector system of our lab and other vectors using the adapter-ligation method.

Please select the vector system for construction of sgRNA expression cassette:

The vector system of YG Liu Lab

Another vector system (use the adapter ligation method).

Reference:

Ma X, Zhang Q, Zhu Q, et al., A robust CRISPR/Cas9 system for convenient high-efficiency multiplex genome editing in monocot and dicot plants.
2015, Mol. Plant, 8 (8), 1274-1284. [Link]

Ma X, Liu Y-G*. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol., 2016, 115, 31.6.1-31.6.21. [Link]

Please enter your target sequence (20 nt) without PAM. [..demo]

ID	Target sequence of CRISPR/Cas9 (5'-3')	Promoter	Method	Primers(5'-3')	Tm
1
2

(Please use IE9 or a higher version of Internet Explorer (IE10, IE11), or "Firefox", "Chrome" to perform the software)