primerDesign-A: design specific PCR primers to amplify the genomic DNA containing the target sites

Step1 - select the target genome

Target (reference) genome:
* If the reference genome sequence is not available, you can select a close relative species or "None".

Step2 - PCR template

Please enter the PCR template sequence containing the target site(s). example

Or input your gene locus ID:
		example

Step3 - target site/marker setting

Please enter your target (20-24 nt) or marker (15-30 nt) sequences and the position of primers

* If your amplicon contains two or more target sites, please input the first and the last target site sequence

First target/marker sequence:
Last target/marker sequence:

	Min	Max
Forward primer position (upstream of first target):
Reverse primer position (downstream of last target):