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offTarget: predict the potential off-target sites of Cas9/Cpf1 RNA-guided endonucleases
PAM type:
SpCas9 from Streptococcus pyogenes: 5'-NGG-3'
FnCpf1 from Francisella: 5'-TTN-3'
AsCpf1 from Acidaminococcus or LbCpf1 from Lachnospiraceae: 5'-TTTN-3'
User defined
*
Or define your own PAM:
PAM sequence:
PAM position:
3'
5'
Guide length (nt):
Target (reference) genome:
Oryza sativa japonica (Nipponbare, IRGSP1.0)
Oryza sativa japonica (Nipponbare, MSU7.0)
Oryza sativa Indica (93-11, ASM465v1)
Oryza sativa indica (MH63RS3)
Oryza sativa indica (ZS97RS3)
Oryza sativa indica (MH63)
Oryza sativa indica (ZS97)
Oryza sativa indica (Shuhui498, R498)
Oryza sativa japonica (Zhonghua 11, ZH11, RiceRC)
Oryza sativa japonica (Kongyu131, KY131, RiceRC)
Oryza sativa japonica (02428, RiceRC)
Arabidopsis thaliana (TAIR10)
Arabidopsis lyrata (v.1.0)
Ananas comosus v3 (Pineapple)
Apple (GDDH13 Version 1.1)
Brachypodium distachyon (v1.0)
Brassica napus (Genoscope, v5)
Brassica rapa (IVFCAASv1)
Brassica oleracea var. oleracea (NCBI)
broccoli (06-9-28)
Camelina sativa (false flax, NCBI)
Cyanidioschyzon merolae (ASM9120v1)
Cucumber (ChineseLong v2, cucurbitgenomics)
Cucurbita moschata (Rifu), CuGenDB v1
Glycine max (v1.0)
Gossypium hirsutum (v1.1, Upland cotton, NBI-AD1)
Hordeum vulgare (ASM32608v1)
Kiwifruit (KIR)
Dimocarpus longan
Medicago truncatula (MedtrA17_4.0)
Musa acuminata (MA1)
Mguttatus_256_v2.0, Mimubase
Morus notabilis (NCBI)
N. tabacum K326 Sierro (Tobacco, Sol Genomics)
Solanum tuberosum (SolTub_3.0)
Solanum tuberosum (Potato, A6-26)
Physcomitrella patens (PhytozomeV11)
Populus trichocarpa (JGI2.0)
Prunus persica (Prupe1_0)
Prunus persica V2 (Prupe V2, GDR)
Poplar_84k (poplar_84k_genome, CNSA)
Setaria italica (JGIv2.0)
Solanum lycopersicum (SL2.50)
Sorghum bicolor (Sorbi1)
Theobroma cacao
Vitis vinifera (IGGP_12x)
Zea mays (AGPv4)
Zea mays (B73_v5)
Zoysia japonica (Zoysia Genome Database, r1.1)
Zoysia matrella (Zoysia Genome Database, r1.1)
Zoysia pacifica (Zoysia Genome Database, r1.1)
Human (GRCh38.p10)
Mouse (GRCm38.p5)
Zebrafish (GRCz10)
Drosophila melanogaster (FlyBase; Ensemble BDGP6)
Caenorhabditis elegans (WormBase; Ensembl WBcel235)
Arabidopsis thaliana mitochondrion (TAIR10)
Oryza sativa mitochondrion (Nipponbare)
Zea mays mitochondrion (B73v2)
Human mitochondrion (GRCh38)
Mouse mitochondrion (GRCm38)
Zebrafish mitochondrion (GRCz10)
Arabidopsis thaliana chloroplast (TAIR10)
Oryza sativa chloroplast (Nipponbare)
Zea mays chloroplast (B73v2)
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