Document for the decoding program

Task submission page:

1. Reference sequence (in FASTA or text format) is the wild-type sequence containing the target site(s) and flanking sequences.

2. The sequence chromatogram file in “ab1” format is uploaded using the “Browse” button.

3. “Anchor sequence” indicates the single-peak sequence bordering the superimposed peaks in the sequencing chromatogram. “Degenerate sequence” indicates the superimposed parts downstream of the anchor sequence. The degenerate sequence is used as a query to search against the reference sequence.

Result display page:

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Liu W., Xie X., Ma X., Li J., Chen J., and Liu Y.-G. 2015. DSDecode: A Web-based Tool for Decoding of Sequencing Chromatograms for Genotyping of Targeted Mutations. Mol. Plant (DOI:

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