DSDecodeM: An updated web tool for rapid decoding of multiple superimposed sequencing chromatograms
Sequence-specific nuclease-based genome targeting systems, i.e., TALENs and CRISPR/Cas9, often cause uniform biallelic and heterozygous mutations in diploid organisms. Direct sequencing of PCR products containing such mutations results in superimposed sequencing chromatograms. The Degenerate Sequence Decode (DSDecode) program serves to decode automatically sequencing chromatograms with biallelic, heterozygous, and homozygous mutations into allelic sequences.
A PC-version of this tool, DSDecodeML, is available upon requested.
Important attention: An alternative website (http://www.ygliulab.club/dsdecode/) of DSDecode is available, please favorite it in case that the current site may be temporarily closed sometimes.
For testing the program, five sequencing ab1 files and the corresponding reference are provided. Click download to obtain the test files.
Xie X, Ma X, Zhu Q, Zeng D, Li G, Liu Y-G. 2017. CRISPR-GE: A Convenient Software Toolkit for CRISPR-Based Genome Editing. Mol. Plant 10(9):1246-1249. (DOI: http://dx.doi.org/10.1016/j.molp.2017.06.004)
Liu W., Xie X., Ma X., Li J., Chen J., and Liu Y.-G. 2015. DSDecode: A Web-based Tool for Decoding of Sequencing Chromatograms for Genotyping of Targeted Mutations. Mol. Plant 8(9):1431-1433. (DOI: http://dx.doi.org/10.1016/j.molp.2015.05.009)
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