Document for the decoding program
Task submission page:
1. Reference sequence (in FASTA or text format) is the wild-type sequence containing the target site(s) and flanking sequences.
2. The sequence chromatogram file in “ab1” format is uploaded using the “Browse” button.
3. “Anchor sequence” indicates the single-peak sequence bordering the superimposed peaks in the sequencing chromatogram. “Degenerate sequence” indicates the superimposed parts downstream of the anchor sequence. The degenerate sequence is used as a query to search against the reference sequence.
Result display page:
If you have any suggestion or question, please contact us by email: skl-cusa@scau.edu.cn; or you can click the "Contact us" at the bottom of page.
Liu W., Xie X., Ma X., Li J., Chen J., and Liu Y.-G. 2015. DSDecode: A Web-based Tool for Decoding of Sequencing Chromatograms for Genotyping of Targeted Mutations. Mol. Plant (DOI: http://dx.doi.org/10.1016/j.molp.2015.05.009)
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